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. 2019 Sep 6;11(9):1315. doi: 10.3390/cancers11091315

Figure 3.

Figure 3

TREM2 suppresses the Wnt/β-catenin signaling pathway in HT29 cells. (A) The indicated mRNA expression levels were analyzed by RT-PCR, (B) and the expression levels of Wnt/β-catenin signaling-related proteins were measured by Western blot in HT29 cells treated with H-Ig or T-Ig (2 μg/mL) for 24 h. (C) Western blot analysis was performed using protein from HT29 cells transfected with plasmids containing the TREM2 gene or vector. The GSK-3β inhibitor LiCl (1 μM) was added for 10 min before analysis. The relative protein expression was quantitatively analyzed by densitometry (lower panel). (D) The expression levels of the indicated proteins in cells isolated from the large intestine of WT littermate and TREM2-TG mice were determined by Western blot. (E) TCF4 luciferase activity was determined in HT29 cells treated with H-Ig or T-Ig (2 μg/mL) for 24 h. The data are representative of three independent experiments (** p < 0.01, *** p < 0.001).