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. 2019 Sep 5;20(18):4369. doi: 10.3390/ijms20184369

Figure 4.

Figure 4

DUSP5 inhibits extracellular-signal-regulated kinase (ERK) signal and leads to ER stress-induced hepatocyte death. (A) Immunoblotting for cleaved caspase-3 and p-ERK. AML12 cell were transfected with MOCK or DUSP5-overexpressing vector (Myc-flag-tagged DUSP5) for 24 h. * p < 0.05 versus MOCK group (n = 3). Asterisk presents the band of endogenous DUSP5. (B) Immunoblotting for cleaved caspase-3 in AML12 cells transfected with control or DUSP5 siRNA, followed by the treatment with TM. ** p < 0.01 versus TM + si Control group (n = 3). (C) Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay in AML12 cells treated with vehicle or TM for 24 h, after transfection with control or DUSP5 siRNA. Values are the means ± standard error of mean (SEM) of 4 separate experiments (each performed in triplicate).