Figure 2.

Gene expression of mannosidases (MANs) (A) and N-acetyl glucosaminyltransferases (MGATs) (B) in activated T cells. Total RNA was extracted from activated T cells and mRNA expression levels were analyzed by qPCR. Results of the relative expression were normalized to the expression levels of the RPL27 housekeeping gene (log₂ transformation, ΔCt). Gene names and primer sequences are listed in Table 2 and Table 4, respectively. Upper and lower quartiles and whiskers of boxes extend to the minimum and maximum values, and the band inside the box is the median. Statistical analysis was performed using an unpaired Student’s t-test, where * p < 0.05; SLE: n = 18, and healthy controls: n = 19.