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. 2019 Aug 30;10(9):666. doi: 10.3390/genes10090666

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Particle picking using EMAN2 [29], Scipion [34], and SuperCryoEMPicker. (a) Manually selected reference particle of the beta-galactosidase [30] image for Scipion [34]; (b) zoom-in view of some manually selected reference particles for the beta-galactosidase [30] image for Scipion [34]; (c) final reference particles of beta-galactosidase manually selected for Scipion [34]; (d) all particle picking results of Scipion [34] trained on 40 manual reference particles on the image of the beta-galactosidase; (e) EMAN2 [29] autopicking results based on different manually selected training samples in the first tested image of the beta-galactosidase dataset [30]; (f) manually selected reference particles of beta-galactosidase [30] for EMAN2 [29]; (g) particle picking results of SuperCryoEMPicker based on SP-IBC clustering; (h) particle picking results of SuperCryoEMPicker based on SP-k-means clustering; (i) particle picking results of SuperCryoEMPicker based on SP-FCM clustering.

Particle picking using EMAN2 [29], Scipion [34], and SuperCryoEMPicker. (a) Manually selected reference particle of the beta-galactosidase [30] image for Scipion [34]; (b) zoom-in view of some manually selected reference particles for the beta-galactosidase [30] image for Scipion [34]; (c) final reference particles of beta-galactosidase manually selected for Scipion [34]; (d) all particle picking results of Scipion [34] trained on 40 manual reference particles on the image of the beta-galactosidase; (e) EMAN2 [29] autopicking results based on different manually selected training samples in the first tested image of the beta-galactosidase dataset [30]; (f) manually selected reference particles of beta-galactosidase [30] for EMAN2 [29]; (g) particle picking results of SuperCryoEMPicker based on SP-IBC clustering; (h) particle picking results of SuperCryoEMPicker based on SP-k-means clustering; (i) particle picking results of SuperCryoEMPicker based on SP-FCM clustering.