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. 2019 Sep 11;8(9):1069. doi: 10.3390/cells8091069

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The expressions of tissue repair-related genes were changed in isolated cholestatic hepatocyte. (A) The detection of Alb and CD31, Laminin, Nestin on cholestatic hepatocyte smear. Scale bars, 50 μm. (B) The mRNA expressions of representative tissue repair-related genes were examined in isolated normal and cholestatic livers. (C) Western blot was employed to study the protein level of tissue repair-related genes. (D) Isolated normal hepatocytes were cultured with 100 ng/mL LPS and the mRNA expression of Laminin, E-cadherin and Cx43 were detected by qPCR. (E) Pathway analysis of top 200 high expressed genes of BDL-6. Data are presented as the means ± SEM. *p < 0.05 vs. control (n = 7 for each group in Figure 5B, n = 3 for each group in Figure 5D).