Figure 2.

Cell proliferation and cell cycle assays in LUAD cell lines with ectopic expression of miR-143-5p and miR-143-3p. (A) Cell proliferation was assessed using XTT assays. Data were measured 72 h after transfection with miRNAs. Cell proliferation was suppressed by miR-143-5p and miR-143-3p in H1299 cells (* p < 0.001), but it was not affected by miR-143-3p transfection into A549 cells. (B,C) Cell cycle assays by flow cytometry showed that G₂/M phase arrest was induced following miR-143-5p transfection into A549 and H1299 cells. In contrast to miR-143-5p transfection, G₀/G₁ phase arrest was detected by miR-143-3p transfection.