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. 2019 Sep 11;20(18):4482. doi: 10.3390/ijms20184482

Figure 7.

Figure 7

Expression of MCM4/MCM4 was directly regulated by miR-143-5p in LUAD cells. (A) Expression of MCM4 mRNA was significantly reduced by miR-143-5p transfection into A549 and H1299 cells (72 h after transfection). Expression of GAPDH was used as an internal control. (B) Expression of MCM4 protein was reduced by miR-143-5p transfection into LUAD cells (72 h after transfection). Expression of GAPDH was used as an internal control. (C) TargetScanHuman database analyses predicted two putative miR-143-5p binding sites in the 3′-UTR of MCM4. (D) Dual luciferase reporter assays showed that luminescence activities were reduced by cotransfection with wild-type (miR-143-5p binding site) vectors and miR-143-5p in LUAD cells. Normalized data were calculated as Renilla/firefly luciferase activity ratios (* p < 0.001).