Figure 4.

H2B-EGFP NG2-dsRed mAR mouse models to study pericytes dynamics during the sprouting process. (A) Time-lapse widefield microscopy analysis of H2B-EGFP NG2-dsRed mARs. White arrow indicate a pericyte that undergoes cell division after 5 h from the beginning of the experiment, generating the two daughter cells marked with blue arrows. (Scale bar: 50 $\mathsf{\mu }$m.) (B) For each cell in a sprout, we verified if it appeared on the sprout coming out of the ring (recruited) or if it divided on the sprout (divided). Each dot represents a single sprout, with green dots for endothelial cells and red dots for pericytes. (C) To measure the recruitment rate of cells on the sprouts, we measured the number of cells at the end of each sprouting assay (each dot represents a sprout) and measured the number of cells during the time of the experiment. (D) Each line represents a division event of a pericyte. Red and blue trajectories are projections of the trajectories of the daughter cells on the direction of the sprout, where the origin has been defined as the coordinate of the mother cells at the frame preceding mitosis. (E) Plot of the distance between daughter cells as a function of time.