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. 2019 Sep 11;20(18):4494. doi: 10.3390/ijms20184494

Table 1.

Primer sequences used in this study.

Genes Forward Primer (5′–3′) Reverse Primer (5′–3′)
Gene Cloning
LjaMYB12 ATGGGGAGAGCACCATGCT CTAAGAGAGCAGCCATGCAAC
Subcellular Localization Assay
LjaMYB12 GGGGTACCATGGGGAGAGCACCATGCT CGGGATCCAGAGAGCAGCCATGCAACC
EGFP CGGGATCCATGGTGAGCAAGGGCGAG ACGCGTCGACTTACTTGTACAGCTCGTCATGC
Transactivation Analysis
LjaMYB12 GGAATTCCATATGATGGTGAGCAAGGGCGAG ACGCGTCGACCTAAGAGAGCAGCCATGCAAC
Arabidopsis Transformation
LjaMYB12 CGGGATCCATGGGGAGAGCACCATGCT ACGCGTCGACCTAAGAGAGCAGCCATGCAAC
qRT-PCR
LjaMYB12 CTTTGAAGGGAATGATGAGG TTGCTTCTCGCTGTCTCC
LjActin TGCTGGATTCTGGTGATGGT ATTTCCCGCTCTGCTGTG
AtPAL ATCGAAGTGATCCGTTACGC ACTCCGATTGGTGTTCCTTG
At4CL CGCAAACCCTTTCTTCACTC ACTCCGTCGTCGTTTTGAAG
AtCHS TGAGAACCATGTGCTTCAGG CAGATGCATGTGACGTTTCC
AtCHI TTTGTACCGTCCGTCAAGTC CAATGACGGTGAAGATCACG
AtF3H TCAGATCGTTGAGGCTTGTG ATGTCGAAACGGAGCTTGTC
AtF3′H CCTCCACCTCCGACTAGGGT TGCTCGGCCACGGATTTA
AtFLS CAACATTCCGAGGTCCAACG TCTTCGTCGGGATCGCTTAG
AtDFR GTCGGTCCATTCATCACAAC TGAGCGTTGCATAAGTCGTC
AtANS TCAAGAAAGCCGGAGAAGAG TTGTCCACTCGCGTTGTTAG
AtACT2 ACCCGATGGGCAAGTCATC CGAGGGCTGGAACAAGACTTC