Gene Cloning
|
LjaMYB12
|
ATGGGGAGAGCACCATGCT |
CTAAGAGAGCAGCCATGCAAC |
Subcellular Localization Assay
|
LjaMYB12
|
GGGGTACCATGGGGAGAGCACCATGCT |
CGGGATCCAGAGAGCAGCCATGCAACC |
EGFP
|
CGGGATCCATGGTGAGCAAGGGCGAG |
ACGCGTCGACTTACTTGTACAGCTCGTCATGC |
Transactivation Analysis
|
LjaMYB12
|
GGAATTCCATATGATGGTGAGCAAGGGCGAG |
ACGCGTCGACCTAAGAGAGCAGCCATGCAAC |
Arabidopsis Transformation
|
LjaMYB12
|
CGGGATCCATGGGGAGAGCACCATGCT |
ACGCGTCGACCTAAGAGAGCAGCCATGCAAC |
qRT-PCR
|
LjaMYB12
|
CTTTGAAGGGAATGATGAGG |
TTGCTTCTCGCTGTCTCC |
LjActin
|
TGCTGGATTCTGGTGATGGT |
ATTTCCCGCTCTGCTGTG |
AtPAL
|
ATCGAAGTGATCCGTTACGC |
ACTCCGATTGGTGTTCCTTG |
At4CL
|
CGCAAACCCTTTCTTCACTC |
ACTCCGTCGTCGTTTTGAAG |
AtCHS
|
TGAGAACCATGTGCTTCAGG |
CAGATGCATGTGACGTTTCC |
AtCHI
|
TTTGTACCGTCCGTCAAGTC |
CAATGACGGTGAAGATCACG |
AtF3H
|
TCAGATCGTTGAGGCTTGTG |
ATGTCGAAACGGAGCTTGTC |
AtF3′H
|
CCTCCACCTCCGACTAGGGT |
TGCTCGGCCACGGATTTA |
AtFLS
|
CAACATTCCGAGGTCCAACG |
TCTTCGTCGGGATCGCTTAG |
AtDFR
|
GTCGGTCCATTCATCACAAC |
TGAGCGTTGCATAAGTCGTC |
AtANS
|
TCAAGAAAGCCGGAGAAGAG |
TTGTCCACTCGCGTTGTTAG |
AtACT2
|
ACCCGATGGGCAAGTCATC |
CGAGGGCTGGAACAAGACTTC |