Figure 8.

Effect of diallyl trisulfide (DATS) on the superoxide dismutase 2 (SOD2) expression induced by ischemia/reperfusion (IR) at seven days. Animals were subjected to 1 h of ischemia and seven days of reperfusion and were administered with four doses of DATS (15 mg/kg, i.p.) every 24 h, starting 5 min before the onset of reperfusion. Brains were collected and fixed at seven days after the first dose of DATS, and coronal sections (5 μm) were obtained. Immunohistochemistry was performed to identify the expression of SOD2 in the striatum and cortex. (A) Representative images of immunohistochemistry at 40× are shown. Analysis of all images is presented as the area of positive mark to SOD2 (µm²)/cell in the striatum (B) and cortex (C). Black arrows indicate SOD2-positive cells. Scale bars represent 50 μm. Data are expressed as mean ± SEM of 3–6 animals per group. ^a p < 0.001 vs. CT group, ^b p < 0.001 vs. CT group and ^c p < 0.001 vs. IR group.