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. 2019 Sep 18;20(18):4621. doi: 10.3390/ijms20184621

Figure 1.

Figure 1

Effects of risperidone (RIS) against transient ischemia (TI) injury under uncontrolled body temperature (UBT) condition. (A) Changes in body temperature under UBT condition for 12 h after TI. Body temperature is significantly low in the TI + 10 mg/kg RIS group compared to the TI + vehicle group. White arrows indicate times of RIS treatment. The bars indicate the means ± SEM, n = 7/group, two-way analysis of variance (ANOVA) with a post-hoc Bonferroni’s multiple comparison test (* p < 0.05 vs. sham + vehicle group; # p < 0.05 vs. TI + vehicle group). (B) Effects of RIS on NeuN+ and F-J B+ cells in the CA1 under UBT condition after TI. In the sham + vehicle group, CA1 pyramidal neurons are well stained with NeuN; however, no F-J B+ CA1 pyramidal cells are found. In the TI + vehicle group, a few NeuN+ cells (arrows) are shown in the stratum pyramidale (SP) 5 days after TI; however, the distribution of NeuN+ cells in the TI + RIS group is similar to that in the sham + vehicle group. In the TI + vehicle group, many F-J B+ cells (asterisks) are detected in the SP 5 days after TI, and many F-J B+ CA1 pyramidal cells (asterisks) are detected; however, in the TI + RIS group, RIS produces a dose-dependent increase in the number of NeuN+ CA1 pyramidal neurons, and a dose-dependent decrease in the number of F-J B+ CA1 pyramidal cells 5 days after TI. CA1, cornu ammonis 1; CA3, cornu ammonis 3; DG, dentate gyrus; SO, stratum oriens; SR, stratum radiatum. Scale bar = 50 μm. Note histograms of quantitative analyses of NeuN+ and F-J B+ cells in all the groups, as shown (C) and (D). The bars indicate the means ± SEM, n = 7/group, two-way analysis of variance (ANOVA) with a post-hoc Bonferroni’s multiple comparison test (* p < 0.05 vs. sham + vehicle group; # p < 0.05 vs. TI + vehicle group).