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. 2019 Sep 13;20(18):4531. doi: 10.3390/ijms20184531

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The ADORA3 antagonism does not affects caspase 3 activation in the kidney of diabetic rats. The levels of cleaved caspase 3 in the kidney sections from experimental groups were assayed by immunohistochemistry. Representative images of cleaved caspase 3 distribution in kidney cortex are shown in (A). Original magnification 400×. The graph in (B) depicts the quantitative analysis of caspase 3 staining on 10 microscopic fields per slice from animals belonging to each experimental group using the UN-SCANIT 2.0 software. n = 5 in each group. *, p < 0.05 vs. control group. In (C) is shown the western blot analysis of cleaved caspase 3 in total kidney protein extracts from experimental groups. β-actin was used as the loading control.