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. 2019 Aug 27;8(9):981. doi: 10.3390/cells8090981

Figure 2.

Figure 2

AC9 is required for sympathetic stimulation of KCNQ1 phosphorylation in vivo. Membranes were prepared from IKs-WT versus IKs-AC9KO heart. (A) AC activity was measured under basal conditions and upon stimulation with 300 nM Gαs (n = 3, performed in duplicate or triplicate). Data are shown as mean +/− SD. Statistics: One Way Analysis of Variance followed by Tukey comparison method for Gαs-stimulated AC activity; * p < 0.05 for WT compared to IKs and IKs-AC9KO. (B) IKs and IKs-AC9KO mice were injected with saline or isoproterenol (2 μg/g body weight, IP). Animals were sacrificed 5 min later and heart tissue was harvested. Cardiac extracts were prepared in the presence of phosphatase inhibitors. Equal protein supernatants were subjected to WB analysis; the ratio of phosphoprotein to total KCNQ1 was quantitated and normalized to IKs-AC9KO control. Data are shown as mean +/− SEM, with individual experimental data points in red. Statistics: Two-way Analysis of Variance followed by a Bonferroni multiple comparison method. n = 5, ** p < 0.001.