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. 2019 Sep 14;20(18):4561. doi: 10.3390/ijms20184561

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phenotype characteristics of hUC-MSCs after 72 h of culture on the GO and rGO substrates. The hUC-MSCs were stained with specific monoclonal antibodies conjugated with fluorochromes (CD45-FITC, CD90-PE, CD105-PE) and analyzed using the LSR Fortessa flow cytometer. The representative histograms (for TCPS, panel A), as well as the charts indicating the expression level of the selected antigen (for the GO and rGO surfaces, panel B), are presented. Legend: GO-sf-1: small flakes/thick layer; GO-sf-2: small flakes/thin layer; GO-lf-1: large flakes/thick layer; GO-lf-2: large flakes/thin layer; rGO-hr-1: high reduction level/thin layer; rGO-hr-2: high reduction level/thick layer; rGO-lr-1: low reduction level/thin layer; rGO-lr-2: low reduction level/thick layer; Control: tissue culture plastic surface (TCPS).