Figure 3.

HDACs 1, 2, and 6 account for the synergism between Mocetinostat and LMK-235 in PDAC cells. HDACs 1, 2, and 3 (A,B) or HDACs 4, 5, and 6 (C) were knocked down (KD) in MiaPaCa-2 and/or Capan-1 cells by transfection with ONTARGETplus SMARTPool siRNAs targeting specific HDACs either individually or in combination, as indicated. Control cells were treated with non-targeting (N.T.) siRNA and, 24 h after transfection, cells were treated with various concentrations of LMK-235 (A,B) or Mocetinostat (C) and subjected to MTT assay after 72 h of drug treatment. (i) Representative graph of the effect of various concentrations of LMK-235 or Mocetinostat on the relative number of viable cells (absorption at 570 nm) following knockdown of the indicated HDACs (mean ± s.d. of six technical replicates for each condition). (ii) ED₅₀ values for LMK-235 (A,B) or Mocetinostat (C) in cells transfected with the indicated siRNAs (mean ± s.d. for three biological replicates).