Table 1.
List of identified protein spots.
| Spot No. | Protein Name | Accession number | Score (Coverage) |
Mw (kDa)/pI | Function |
|---|---|---|---|---|---|
| 1 | Apo A1 | P02647 | 215 (61%) | 30.76/5.56 | Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). |
| 2 | Apo J (clusterin) | P10909 | 38 (19%) | 49.34/6.27 | Prevents stress-induced aggregation of blood plasma proteins. |
| 3 | α2-HS-glycoprotein | P02765 | 72 (28%) | 40.10/5.43 | Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. |
| 4 | α1-B-glycoprotein | P04219 | 130 (43%) | 54.81/5.65 | . |
| 5 | Haptoglobin α chain | P00738 | 80 (51%) | 13.74/6.10 | Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. |
| 6 | Fibrinogen-γ-A chain | P02679 | 158 (58%) | 50.82/5.70 | Together with fibrinogen-α (FGA) and fibrinogen-β (FGB), polymerizes to form an insoluble fibrin matrix. |
| 7 | Fibrinogen-γ-B chain | P02679 | 156 (56%) | 52.11/5.37 | Together with fibrinogen-α (FGA) and fibrinogen-β (FGB), polymerizes to form an insoluble fibrin matrix. |
| 8 | antithrombin | P01008 | 182 (58%) | 53.04/6.32 | Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. |
| 9 | Fibrinogen- | P02675 | 225 (62%) | 56.58/8.54 | Cleaved by the protease thrombin to yield monomers which, together with fibrinogen-α (FGA) and fibrinogen-γ (FGG), polymerize to form an insoluble fibrin matrix. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. |
| 10 | Serotransferrin | P02787 | 93 (37%) | 74.28/6.63 | Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. |
| 11 | α1-antiproteinase | P01009 | 148 (47%) | 46.88/5.37 | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. |
| 12 | Immunoglobulin α2 heavy chain | P01876 | 133 (37%) | 54.82/6.02 | Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. |
| 13 | Haptoglobin β chain | P00738 | 126 (46%) | 38.87/6.26 | Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. |
| 14 | α2-microglobulin | P01023 | 172 (25%) | 164.6/6.00 | Is able to inhibit all four classes of proteinases by a unique ‘trapping’ mechanism. |
| 15 | Complement C3 | P01024 | 107 (24%) | 188.57/6.02 | C3 plays a central role in the activation of the complement system. |
| 16 | Fibrinogen-α | P02671 | 127 (38%) | 94.97/5.70 | Cleaved by the protease thrombin to yield monomers which, together with fibrinogen-β (FGB) and fibrinogen-γ (FGG), polymerize to form an insoluble fibrin matrix. |
| 17 | ApoE | P02649 | 82 (43%) | 36.15/5.65 | APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids |
| 18 | Plasminogen | P00747 | 72 (23%) | 90.57/7.04 | Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. |
Database: NCBIprot 20180429 (152462470 sequences; 55858910152 residues).