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. 2019 Sep 15;8(9):1089. doi: 10.3390/cells8091089

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative immunofluorescent photomicrographs showing the protein expression levels of differentiation (filaggrin) and desmosomal (desmoglein-1) protein markers in control RHSE and FTRHSP under different treatment conditions versus fisetin or Vit-D₃-treated FTRHSP tissues. Results are representative of three independent experiments each performed in quadruplicate and comparing control RHSE vs. FTRHSP treated groups. Data green (Dsgl-1); red (filaggrin), blue (DAPI) and mixed is merged representation. Dsgl-1 = desmoglein-1. Scale bar = 20 μm.