Figure 5.

Lycopene inhibits epidermal growth factor receptor (EGFR) transactivation. (A) The cells were treated with 2 μM lycopene for the indicated time periods. The protein levels of p-EGFR and EGFR were examined by Western blot analysis. (B) The cells were treated with the indicated concentrations of lycopene for 2 h. Whole-cell extracts were subjected to immunoprecipitation (IP) with anti-phosphorylated tyrosine (p-Tyr) antibody, followed by subsequent Western blot analysis (WB) with anti-EGFR antibody (upper panel). The input of EGFR and p-Tyr was determined by Western blot analysis and served as control for the amounts of EGFR and p-Tyr (lower panel). The cells treated with a vehicle tetrahydrofuran without lycopene were expressed as “none”.