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. 2019 Sep 19;11(9):2254. doi: 10.3390/nu11092254

Figure 3.

Figure 3

Effect of ovotransferrin on RANKL-induced MAPK activation. RAW 264.7 cells were pretreated with ovotransferrin (1–1000 μg/mL) for 30 min prior to stimulation with RANKL (100 ng/mL). Then, RAW 264.7 cells were treated with both RANKL and ovotransferrin for 4 h. Whole cell lysates were used for Western blot analysis of (A) p-p44/42 Erk1/2 (Thr 202/Tyr 204)/p44/42 Erk1/2, (B) p-p38 (Thr 180/Tyr182)/p38, and (C) p-JNK (Thr 183/Tyr 185)/JNK. (D) All bands are shown. The results are expressed as means ± SEM representative of 4 independent experiments. Means with different letters indicate p < 0.05.