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. 2019 Sep 19;11(9):2254. doi: 10.3390/nu11092254

Figure 4.

Figure 4

Effect of ovotransferrin on RANKL-induced expression of proteins involved in osteoclastogenesis. RAW 264.7 cells were pretreated with ovotransferrin (1–1000 μg/mL) for 2 h prior to stimulation with RANKL (100 ng/mL). Then, RAW 264.7 cells were co-cultured with RANKL and ovotransferrin for 12 h. Whole cell lysates were used for Western blot analysis of (A) TRAF6, (B) cFos, (C) NFATc1, and (D) cathepsin K. (E) All bands are shown. The results are expressed as means ± SEM representative of 4 independent experiments. Means with different letters indicate p < 0.05.