Figure 5.

Effect of ovotransferrin on RANKL-induced bone resorption in vitro. RAW 264.7 was cultured onto fluoresceinated calcium phosphate (CaP)-coated plate and stimulated with RANKL (100 ng/mL) for 6 days. After 1 day for adhesion, cells were treated with ovotransferrin (1–1000 μg/mL) and RANKL together for 6 days. (A) The fluorescence intensity of resorbed CaP during 6-day treatment. * p < 0.05. (B) The total resorbed CaP after 6 days. (C) The total area of resorption pits after 6 days. (D) Image of resorption area. All images were captured under 10× magnification. The results are expressed as means ± SEM representative of 4 independent experiments. Means with different letters indicate p < 0.05.