Figure 3.

Effect of 6SL on E2F transcription factor 1 (E2F1)-relative and cell cycle protein expression in TP-induced BPH rat model. (A) The manifestation of retinoblastoma protein (pRb) in prostatic tissues from rats was shown by immunohistochemistry. The immunoblotting images and quantitative analysis show the protein expression of (B) pRb, E2F1, and (C) cyclin A, cdk2, cyclin D1, Cdk4. The densities of proteins were calculated using ImageJ Software. Relative protein level represents densitometric values of each protein as ratio to β-actin. Values with different letters indicate significant differences, p < 0.05.