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. 2019 Sep 5;20(18):4357. doi: 10.3390/ijms20184357

Figure 3.

Figure 3

Dapagliflozin reduces glucose transporter (GLUT)-induced expression of the receptor for advanced glycation end products (RAGE), nicotinamide adenine dinucleotide phosphate reduced form oxidase 4 (NOX4) and nicotinamide adenine dinucleotide phosphate reduced form (NADPH) oxidase subunit (p67-phox) levels in the lenses of rats with fructose-induced type 2 diabetes mellitus (DM). (A–B) Representative fluorescence images of GLUT5- and RAGE-expressing cells (green) and p7-phox and NOX4-expressing cells (red) in the lens with or without a systemic administration of fructose or dapagliflozin. Cell nuclei are counterstained with DAPI (blue). (C–E) Quantitative immunoblotting analysis demonstrating that the GLUT1, GLUT5, and p67-phox levels in the lenses of rats with fructose-induced type 2 DM were significantly decreased by dapagliflozin administration. Values are presented as the means ± SEMs (n = 6 per group, separate experimental groups in each figure). Scale bar: 20 μm. * p < 0.05; # p < 0.05 vs. Fructose 8 W or 12 W.