Table 1.
Primer sequence for qPCR.
| Genes | Primer Sequence (Forward) | Primer Sequence (Reverse) | Product Size (bp) |
|---|---|---|---|
| Il-6 | GAAGTTCCTCTCTGCAAGAGAC | CCAGAGAACATGTGTAATTAAGC | 178 |
| Il-10 | GCCGGGAAGACAATAACTGC | TTCAGCTTCTCACCCAGGGA | 280 |
| Tnfa | AGCACAGAAAGCATGATCCGCG | AGAAGATGATCTGAGTGTGA | 249 |
| Baff | TCCAGCAGTTTCACAGCGAT | CCGGTGTCAGGAGTTTGACT | 157 |
| Cxcl13 | CACGGTATTCTGGAAGCCCA | AGACAGACTTTTGCTTTGGACA | 231 |
| Myd88 | TGTTCTTGAACCCTCGGACG | TTCTGGCAGTCCTCCTCGAT | 241 |
| Traf3 | CAGCGCACGGCACAGAATTT | CTTGTAGCCTCCTTGCTCCG | 149 |
| Irf5 | CTAGCCTGGATGTGGCATGT | CTCTTTAGCCCAGGCCTTGAA | 314 |
| Nfkb1 | CCACAAGGGGACATGAAGCA | GATGGTACCCCCAGAGACCT | 198 |
| β-actin | AGATCAAGATCATTGCTCCTCCT | ACGCAGCTCAGTAACAGTCC | 174 |
Forward and reverse primer sequence of each target is list in above. Efficiency of each primer set was validated and was comparable to the endogenous house-keeping gene β-actin.