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. 2019 Aug 27;9(9):609. doi: 10.3390/ani9090609

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of over-expression of NTN1 on the proliferation and differentiation of C2C12 cells. (A) Expression of NTN1, KI67, and P21 gene in C2C12 cell proliferation phase. (B) Expression of MCK, MYOG, MYOD, and NTN1 gene in C2C12 cell differentiation phase. The results were represented as mean ± SD. The expression level of genes in NTN1 over-expression C2C12 cells was determined by q-PCR using NC as the control. PEX-3 and PEX-1 were the over-expression plasmid, and the negative control, respectively. * p < 0.05.