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. 2019 Sep 12;11(9):1349. doi: 10.3390/cancers11091349

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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E₂ alters the subcellular localization of Mdm4 in mouse thymocytes. (A–C), WB analysis of whole cell extracts (WCEs) (A) cytosolic extracts (B) and nuclear extracts (C) of thymocytes of Mdm4^Tg15 females treated with a single i.p. dose of physiologic solution or 50 µg/Kg E₂ and sacrificed after 16 h. Laminin and GAPDH were used as LC. Graphs show mean ± SD of MDM4 levels quantified by Alliance V_1607 software (two-tailed unpaired t-test, WCEs: t = 2.443, df = 10, * p = 0.0346, Cytosolic extracts: t = 2.629, df = 10, * p = 0.0252, Nuclear extracts: t = 2.891, df = 9, * p = 0,0179).