Figure 2.

Expression of NLRP1, AIM2, and NLRP3 inflammasomes in peripheral blood mononuclear cells (PBMCs) in response to sulfasalazine (SSZ). The expression of inflammasome-related gene was quantified in PBMCs from healthy donors and people living with HIV. The PBMCs were stimulated with inflammasome agonists (MDP 0.1 μg/mL, ATP 2 mM, and poly(dA;dT) 50 μg/mL) in the presence of 1 mM SSZ during 4 h of incubation or 2 h for ATP-treated cells. qPCR experiments are as indicated: mRNA NLRP1 (A), mRNA AIM2 (B), and mRNA NLRP3 (C). The β-actin gene was used as a constitutive gene to normalize the RNA content. Statistical comparison was performed using a Wilcoxon matched-pairs signed rank one-tailed test with a confidence level of 95%. Significant differences are indicated at the top of the figure (* p < 0.05) (** p < 0.01).