Figure 4.
IL-1β release by peripheral blood mononuclear cells (PBMCs) in response to TLRs agonists and sulfasalazine (SSZ). IL-1β production by PBMCs from healthy donors (A) and people living with HIV (B); these cells were treated in vitro with TLR agonist (LPS 100 ng/mL; poly(I:C) 1 μg/mL; R848 1 μg/mL; Pam2CSK4 40 ng/mL; and CpG-K16 4 μg/mL) in the presence of 1 mM SSZ during 18 h of incubation and were quantified by ELISA. Statistical comparison between groups was performed using a Wilcoxon matched-pairs signed rank one-tailed test with a confidence level of 95%. (+: presence, −: absence).