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. 2019 Sep 11;9(9):474. doi: 10.3390/biom9090474

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mesenchymal stem cells (MSCs) established a prototypical cluster of differentiation (CD) expression profile expected of stem cells independent of Cx43 expression and functional status. (A) Representative immunoblot of Cx43 (green) and GAPDH (red) from control, ODDD patient-derived, and Cx43-/- iPSC-derived MSCs. (B) Scrape-loading transfer of gap junction channel-permeable Lucifer yellow revealed decreased GJIC in Cx43-/- MSCs compared to control cells. ***, p < 0.001 compared to WT. Data represent the standard deviation of the mean of 4–5 independent experiments comprising at least 144 individual measurements for each cell line. (C) Phase contrast and (D) immunofluorescent micrographs of Cx43 (red) and nuclei (Hoechst, blue) in control, ODDD patient-derived and Cx43-/- MSCs. Arrows indicate gap junction plaques between adjacent cells. Scale bar = 10 μm (E) MSCs differentiated from control, ODDD patient-derived and Cx43-/- iPSCs were analyzed by flow cytometry for the cell surface markers CD34, CD45, CD73, and CD105.