Figure 3.

Cx43 was significantly upregulated during adipogenic differentiation of MSCs. (A) MSCs were cultured for up to 14 days under adipogenic conditions before labeling for Cx43 (red), lipid (green; LipidTox), and nuclei (Hoechst, blue). Scale bar = 10 μm. (B) Representative immunoblot of Cx43 (green) and GAPDH (red) in undifferentiated MSCs and MSCs differentiated along the adipogenic lineage for 7 and 14 days. The differential phosphorylation states of Cx43 are noted as P₀, P₁, and P₂. (C) Quantitative analysis of Cx43 levels in control and ODDD patient-derived MSCs before and after 7 and 14 days of adipogenic differentiation. *, p < 0.05; **, p < 0.01 compared to undifferentiated MSCs. Data represent the standard error of the mean of three independent experiments.