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Early passage MSCs differentiate along the adipogenic lineage independent of Cx43 status. Representative images of undifferentiated (passage, P3-P5) and differentiated MSCs after staining for lipid droplets (LipidTox, green), perilipin (red), Cx43 (red), and/or Hoechst (nuclei, blue) after 0, 7, or 14 days of differentiation. (A) Control, (B) ODDD patient-derived, and (C) Cx43-/- MSCs were labeled at 0, 7, or 14 days after in vitro adipogenic differentiation. Scale bar = 10 μm.
