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. 2019 Sep 14;20(18):4560. doi: 10.3390/ijms20184560

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Histological analysis of macrophage infiltration in distal colon of ETBF-infected mice. FFPE distal colonic tissues obtained from mice at day 7 post-infection were stained with anti-F4/80 antibody and counterstained with hematoxylin. Representative images are shown. S, sham; ET, ETBF; Z (30), Zerumbone (30 mg/kg); Z (60), Zerumbone (60 mg/kg). (A) Immunohistochemistry (IHC) for F4/80⁺ cells, ×200 magnification; Scale bar, 100 μm. (B) Median number of F4/80⁺ cells at 200× field. Scatter plot. Horizontal bar, median. * p < 0.05, ** p < 0.01. ns, no statistical significance.