. 2019 Aug 27;9(9):613. doi: 10.3390/ani9090613

Table 1.

Polymerase chain reaction (PCR) primers used in this study.

Gene	Region	Primer Sequence (5′-3′)	Amplicon Size (bp)	Purpose of Primers
FASN	Exon 24-intron 24	F: CTGTCACCTTCCTCACTTGCCCT	390	PCR-SSCP analysis
FASN	Exon 24-intron 24	R: GAGGAGGAATCGGCCAGGATGTT	390	PCR-SSCP analysis
FASN	Exon 34	F: CCCTCTAAAGCCGTCCTCACCA	220	PCR-SSCP analysis
FASN	Exon 34	R: CCAGACCTTCATTTGCCAATCCTC	220	PCR-SSCP analysis
FASN		F: ACAAGACAAGCCCGAGGAG	203	RT-qPCR
FASN		R: TAGCAGGCAGTTCCGAGAG	203	RT-qPCR
β-actin		F: AGCCTTCCTTCCTGGGCATGGA	113	RT-qPCR
β-actin		R: GGACAGCACCGTGTTGGCGTAGA	113	RT-qPCR

bp: base pairs; PCR-SSCP: polymerase chain reaction-single stranded conformational polymorphism; RT-qPCR: reverse transcription-quantitative PCR.