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. 2019 Aug 21;11(9):1971. doi: 10.3390/nu11091971

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of Scenedesmus diets on hepatic, serum, urinary, fecal metabolomes. Data from LC-MS analysis of liver extract, serum, urine and fecal extract were processed by supervised partial least squares-discriminant analysis (PLS-DA) modeling. The associations of metabolites representing each of the three groups of mice (n = 8/group) are shown in the scores plots. Selective metabolite markers correlating with the experimental diets are labeled in the loadings plots. (A) Scores plot of a PLS-DA model on liver extracts. (B) Loadings plot of a PLS-DA model on liver extracts. (C) Scores plot of a PLS-DA model on serum samples. (D) Loadings plot of a PLS-DA model on serum samples. (E) Scores plot of a PLS-DA model on urine samples. (F) Loadings plot of a PLS-DA model on urine samples. (G) Scores plot of a PLS-DA model on fecal samples. (H) Loadings plot of a PLS-DA model on fecal samples. The symbol “↑” in the loadings plot indicates positive correlation with individual treatment.