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. 2019 Sep 6;9(9):453. doi: 10.3390/biom9090453

Table 1.

Scoring procedures in the horizontal bar method.

Serial No. Duration of Rat on Horizontal Bar Score
1. Falling time between 1 and 5 s 1
2. Falling time between 6 and 10 s 2
3. Falling time between 11 and 20 s 3
4. Falling time between 21 and 30 s 4
5. Falling time after 30 s 5
6. The maximum score (5) was allotted if the rat placed one forepaw on the bar support without falling. 5

Note 1: If the experimental animal failed to grasp the bar properly and its was obviously due to error of the experimenter, the test was repeated after a brief rest by the rat to obtain a falling time greater than 5 s. Note 2: If the rat in three different attempts fell before 5 s and it appeared that it was not due to error of the experimenter in placing the rat on the bar, it was recorded and the best score was taken for the data.