Figure 2.

Norvaline reduces the blood–brain barrier (BBB) permeability. BBB integrity was evaluated via Evans blue BBB permeability assay. Homozygous triple-transgenic mice models of Alzheimer’s disease (3×Tg-AD) and wild-type (WT) mice were intraperitoneally injected with Evans blue dye (50 µg/g of body weight). Three hours post-injection, mice were deeply anesthetized, perfused with saline, and dye presence in the brains was measured at OD₆₂₀. (A) Representative images of the control and norvaline-treated brains. (B) The brain homogenates of control and norvaline-treated mice were analyzed spectrophotometrically. The OD values at 620 nm were normalized by division by the weights of the appropriate brains and presented as means ± SEM, ** p < 0.01, n = 6.