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. 2019 Sep 18;20(18):4616. doi: 10.3390/ijms20184616

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Microglia infiltrate 3×Tg-AD mice brains in a perivascular fashion. Representative hippocampal bright-field × 40 micrographs with × 100 insets. Perivascular infiltration with CD-68+ cells of control (A) and norvaline-treated mice (B) hippocampi. Iba1+ microglia in control (C) and norvaline-treated (D) mice. Measurement of the CD-68 immunopositive surface area (E) and stain intensity (F) with ZEN 2.5 revealed a significant effect of the treatment. (G) The relative intensity of perivascular Iba1+ microglia. Student’s unpaired t-test, n = 20, * p < 0.05.