Figure 7.

(A) Representative Western blot protein analysis of the BACE1 and GAPDH proteins after 24 h treatment with AO2 targeting exon 2 at 400 nM in HEK293 cells. FL, full-length; UT, untreated; SCR, scrambled sequence; GAPDH was used as a loading control. The gel images were cropped to highlight the BACE1 protein and the corresponding house-keeping protein control GAPDH. The original images are shown in Figure S7 (Supplementary Information). (B) Densitometry analysis of protein products showed downregulation of BACE1 protein in HEK293 cells in vitro.