Figure 4.

qRT-PCR analysis of tomato (S. lycopersicum cv. Ailsa Craig) LOX gene family members in response to cold stress. Graphical representation of qRT-PCR data of tomato 9-LOX genes and 13-LOX genes in response to cold treatment at 4 °C for up to 24 h. A non-treated control (0 h) was used as calibrator in qRT-PCR data calculation and for defining statistical significance of treatment data points at levels * p < 0.05, ** p < 0.01 and *** p < 0.001. Details are given in Figure S2. A minimum of three biological replicates, where each biological replicate was comprised of two technical replicates, were used for each time point. Housekeeping genes, SlTIP41 and SlUBI3, were used to normalize the expression of target genes.