Figure 5.

qRT-PCR analysis of tomato (S. lycopersicum cv. Ailsa Craig) LOX gene family members in response to drought stress. Graphical representation of 9-LOX genes and 13-LOX genes in response to drought (water withheld for 168 h). A non-treated control (0 h) was used as a calibrator in qRT-PCR data calculation and for defining statistical significance of treatment data points at levels * p < 0.05, ** p < 0.01 and *** p < 0.001. Details are given in Figure S3. A minimum of three biological replicates, where each biological replicate was comprised of two technical replicates, were used for each time point. SlTIP41 and SlUBI3 housekeeping genes were used to normalize the expression of target genes.