Fig. 2.

BBD prevents ethanol-induced hepatocyte oxidative damage. a ROS level in liver was measured by using 2ʹ,7ʹ-dichlorofluorescein diacetate (DCFH-DA) and analysed by the ELx 808 Universal Microplate Reader apply. b Liver and serum MDA levels were determined as indicated methods. Effects of BBD on CYP2E1 gene expression, including c immunoblot analysis and d real-time PCR analysis. Effects of BBD on liver ADH (e), GSH, GPx, SOD, CAT (f). The data presented are from three replicates as mean ± SD. ^#P < 0.05, ^##P < 0.01 compared to control; *P < 0.05, **P < 0.01 compared with NS group; ^{^}P < 0.05. NS normal 0.9% saline, EtOH ethanol, BBD Babao Dan, 0.125 BBD 0.125 mg/kg BBD, 0.25 BBD 0.25 mg/kg BBD, 0.5 BBD 0.5 mg/kg