Fig. 4.

Nrf2 activation contributes BBD-mediated hepatoprotective effect. a Western blotting analysis of Nrf2 and the two transcriptional targets of HO-1 and GCLC expression in response to BBD (0.5 g/kg BW) alone. b Real-time PCR analysis of Nrf2 and the two downstream targets of HO-1 and GCLC transcription reacted to BBD treatment alone. Effects of BBD on related genes expression in c protein and d transcriptional level in liver. Experiments were repeated three times. Data were expressed as mean ± SD; ^#P < 0.05, ^##P < 0.01 compared to control; *P < 0.05, **P < 0.01 compared to NS + EtOH group; ^{^}P < 0.05. EtOH ethanol, 0.125 BBD 0.125 mg/kg BBD, 0.25 BBD 0.25 mg/kg BBD, 0.5 BBD 0.5 mg/kg