Table 1.
Primers designed to amplify the zinc finger 695 alternative splice variants.
| Transcript | Primer Sequence | TM | Amplicon Size |
|---|---|---|---|
| RPL4 | Forward: 5′ CGAATGAGAGCTGGCAAAGGCAAA 3′ | ||
| Reverse: 5′ ACGCCAAGTGCCGTACAATTCATC 3′ | 60 °C | 243 bp | |
| ZNF695_TV1 | Forward: 5′ CTGGAGAGGCTCTTTGTACTTG 3′ | ||
| Reverse: 5′ GATAGGTTAACGTTGGTGGTAGG 3′ | 57.2 °C | 279 bp | |
| ZNF695 TV2/TV3 | Forward: 5′CCTTTGCCTTCTGCCATGAT 3′ | ||
| Reverse: 5′ TTAATTCAGAACTCGGGCTGAC 3′ | 55.2 °C | 212 bp | |
| ZNF695_TV4 | Forward: 5′ GTGGCCTGCAGGGACTATTG 3′ | ||
| Reverse: 5′ TGCAAGAGACATTGCCACATTC 3′ | 56.5 °C | 591 bp | |
| ZNF695_TV5 | Forward: 5′ CTCCCTTGGTCTTGCTATGT 3′ | ||
| Reverse: 5′ TGCAAGAGACATTGCCACATTC 3′ | 54.5 °C | 425 bp | |
| ZNF695_TV6 | Forward: 5′ GTGGCCTGCAGGGACTATTG 3′ | ||
| Reverse: 5′ TTAATTCAGAACTCGGGCTGAC 3′ | 56.5 °C | 638 bp | |
| ZNF695_TV7 | Forward: 5′ CTCCCTTGGTCTTGCTATGT 3′ | ||
| Reverse: 5′ TTAATTCAGAACTCGGGCTGAC 3′ | 54.5 °C | 472 bp |