Table 2.
Recurrence-free survival and overall survival analysis by clinico-pathologic variables and pre- and post-operative ctDNA status
| Pre-operative variable and RFS | ||||||
|---|---|---|---|---|---|---|
| Variable | Univariate analysis |
Multivariate analysis |
||||
| HR | 95% CI | P | HR | 95% CI | P | |
| CA19-9 at diagnosis, <36 U/ml versus >36 U/ml | 1.6 | 0.7–3.5 | 0.31 | |||
| Tumor stage, T3/4 versus T1/2 | 4.4 | 0.6–32.9 | 0.145 | 6.4 | 0.7–57.5 | 0.100 |
| Lymph node status, N1 versus N0 | 1.7 | 0.6–5.0 | 0.318 | 0.8 | 0.2–3.2 | 0.731 |
| Resection margin status, R1 versus R0 | 1.3 | 0.6–3.0 | 0.488 | 2.1 | 0.5–8.7 | 0.325 |
| Lymphvascular invasion status, present versus absent | 2.3 | 0.8–6.8 | 0.130 | 1.1 | 0.3–4.0 | 0.887 |
| Perineural invasion status, present versus absent | 0.8 | 0.3–2.1 | 0.726 | 0.3 | 0.1–1.2 | 0.083 |
| ctDNA status, positive versus negative | 3.9 | 1.4–10.8 | 0.008 | 4.1 | 1.4–12.1 | 0.018 |
| Pre-operative variable and OS | ||||||
| Tumor stage, T3/4 versus T1/2 | 2.9 | 0.4–22.0 | 0.300 | |||
| Lymph node status, N1 versus N0 | 1.0 | 0.3–3.0 | 0.960 | 0.7 | 0.1–3.6 | 0.653 |
| Resection margin status, R1 versus R0 | 1.4 | 0.5–4.0 | 0.533 | 2.0 | 0.6–7.1 | 0.290 |
| Lymphvascular invasion status, present versus absent | 1.3 | 0.4–4.2 | 0.616 | |||
| Perineural invasion status, present versus absent | 0.8 | 0.3–2.4 | 0.715 | |||
| ctDNA status, positive versus negative | 3.6 | 1.0–13.1 | 0.051 | 4.1 | 1.0–16.6 | 0.049 |
| Post-operative variable & RFS | ||||||
| Tumor stage, T3/4 versus T1/2 | 4.4 | 0.6–32.9 | 0.145 | |||
| Lymph node status, N1 versus N0 | 1.7 | 0.6–5.0 | 0.318 | 0.2 | 0.0–1.1 | 0.068 |
| Resection margin status, R1 versus R0 | 1.3 | 0.6–3.0 | 0.488 | 2.4 | 0.6–10.1 | 0.221 |
| Lymphvascular invasion status, present versus absent | 2.3 | 0.8–6.8 | 0.130 | 59.6 | 3.0–1178.8 | 0.007 |
| Perineural invasion status, present versus absent | 0.8 | 0.3–2.1 | 0.726 | 0.1 | 0.0–0.5 | 0.009 |
| ctDNA status, positive versus negative | 5.4 | 1.9–15.2 | <0.0001 | 6.3 | 2.4–16.2 | <0.0001 |
| Post-operative variable & OS | ||||||
| Tumor stage, T3/4 versus T1/2 | 2.9 | 0.4–22.0 | 0.300 | |||
| Lymph node status, N1 versus N0 | 1.0 | 0.3–3.0 | 0.960 | 1.3 | 0.3–7.3 | 0.733 |
| Resection margin status, R1 versus R0 | 1.4 | 0.5–4.0 | 0.533 | 0.6 | 0.2–2.3 | 0.494 |
| Lymphvascular invasion status, present versus absent | 1.3 | 0.4–4.2 | 0.616 | |||
| Perineural invasion status, present versus absent | 0.8 | 0.3–2.4 | 0.715 | |||
| ctDNA status, positive versus negative | 5.5 | 1–17.4 | 0.004 | 7.5 | 2.1–27.7 | 0.002 |