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. 2019 Sep 18;9(22):6690–6705. doi: 10.7150/thno.34520

Figure 2.

Figure 2

iHepLPCs converted back in the hepatic maturation medium. (A) qPCR analyses for the expression of CYP3A4, ALB, AAT, G6PC, CPS1, HNF4a, CK19 and EpCAM during hepatic-differentiation of iHepLPCs in mHMM (passage 30) from day 0 to day 15. Error bars represent s.d.; n = 3. (B) Albumin secretion, AAT secretion, (C) urea production and ammonia elimination after 24 h of culture, measured from supernatants. Error bars represent s.d.; n = 3 (D) Images of iHepLPCs before (left) and after (right) 9 days of differentiation. iHepLPCs-Hep underwent distinct morphological changes. The scale bar is 100 μm. (E) PAS staining with or without diastase and (F) CDCFDA staining of iHepLPCs before (left) and after (right) 9 days of differentiation. arrows indicated functional bile canaliculi, the scale bar is 100 μm. (G) Immunofluorescence staining of albumin, HNF4a in iHepLPCs before (left) and after (right) 9 days of differentiation, the scale bar is 100 μm.