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. 2019 Sep 18;9(22):6690–6705. doi: 10.7150/thno.34520

Figure 3.

Figure 3

3D spherical differentiation promoted further hepatic maturation. (A) Images of iHepLPCs-Hep-3D; the scale bar is 200 μm. (B) qPCR analysis of iHepLPCs-Hep-3D and iHepLPCs-Hep showed that 3D spherical differentiation significantly increased the expression of liver-specific genes. Error bars represent s.d.; n = 3 (C) Comparison of levels of albumin, antitrypsin, (D) urea production and ammonia elimination between freshly isolated PHCs, iHepLPCs-Hep and iHepLPCs-Hep-3D. Error bars represent s.d.*P < 0.05, **P < 0.01, ***P<0.001, ****P<0.0001; n = 3. (E) Metabolic activities of CYPs in 3D-HepLPCs-Hep, PHCs (freshly isolated). The metabolic products of Acetaminophen, OH-Bupropion, OH-Diclofenac, OH-Testosterone and OH-Coumarin Glu were determined by liquid chromatography-tandem mass spectrometry according to standard curves. Error bars represent s.d.; n = 3. (F) Induction of CYP450 activity in iHepLPCs-Hep-3D in response to 72-h stimulation with omeprazole or rifampicin. Omeprazole significantly induced CYP1A2 expression, whereas rifampicin induced CYP3A4 expression. Error bars represent s.d.; *P < 0.05, **P < 0.01; n = 3.