Table 1.
Physicochemical characterization of the NPs
| Loading capacity (% w/w) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Samples | Diameter | ζ Potential (mV) | PDI | NIR | Dox | pIC | R848 | MIP3α |
| NP(NIR)-PEG Denoted as NP(empty) |
187.4 ± 44.7 | -13.9 ± 6.2 | 0.064 | 63.6 ± 1.4 | - | - | - | - |
| NP(NIR+dox)-PEG Denoted as NP(dox) |
185.9 ± 28.2 | -13.5 ± 7.5 | 0.127 | 64.9 ± 0.9 | 13,9 ± 1.8 | - | - | - |
| NP(NIR+pIC+R848+MIP3α)-PEG Denoted as NP(pIC+R848+MIP3α) |
177.3 ± 86.6 | -14.3 ± 4.9 | 0.120 | 61.1 ± 7.8 | - | 47.7 ± 2.6 | 58.4 ± 3.2 | 63.8 ± 5.0 |
| NP(NIR+dox+pIC+R848+MIP3α)-PEG Denoted as NP(dox+pIC+R848+MIP3α) |
177.3 ± 86.8 | -14.3 ± 4.9 | 0.120 | 62.8 ± 5.6 | 6.3 ± 1.1 | 37.9 ± 10.1 | 17.1 ± 3.8 | 63.9 ± 3.8 |
Physicochemical characterization of the PLGA-PEG NPs containing dox and/or different immune adjuvants. The PLGA NPs were characterized by dynamic light scattering and zeta potential measurements. PLGA NPs size and zeta potential data represent the mean value ± SD of 10 readings of one representative batch. The loading capacity of dox and NIR dye was measured by fluorescence method. The loading capacity of pIC, R848 and MIP3α was determined by RP-HPLC analysis. The loading capacity data represent the average value ± SD of batch variation.