Figure 2.
TIMP3 exhibited positive correlation with disease stages and recurrence time in colon cancer. (A) Kaplan-Meier analysis for the influence of TIMP-3 on recurrence time and survival time. (B) Expression level of TIMP3 in colorectal cancer (GSE8671, n = 32, p = 6.7 x 10-9, left panel; GEPIA expression database, n = 257 in cancerous tissue sand n = 159 in normal tissue, right panel). Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. Two-tailed Student's t test was used to determine all P values. (C) TIMP3 expression of three CRC patients in non-tumor part and tumor part tissue. (D) Different colorectal cancer cells were collected followed by western blot for TIMP3 detection. NIH3T3 were loaded as positive control. HUVEC and FHC cells were loaded as normal cells for comparison.