Figure 3. MetS+RAS causes podocyte mitochondrial injury.
A: Representative transmission electron microscopy images of podocytes in all groups. Podocyte mitochondrial density was similarly decreased in MetS, Lean+RAS, and MetS+RAS compared to Lean, and influenced only by ischemia. Mitochondrial area, perimeter, and matrix density were lower in MetS compared to Lean, but the latter further decreased in Lean+RAS and MetS+RAS and was influenced by MetS diet, ischemia, and their interaction. B: Glomerular score was higher in Lean+RAS versus Lean and MetS, further increased in MetS+RAS, and was influenced by MetS diet, ischemia, and their interaction. *p<0.05 vs. Lean, †p<0.05 vs. MetS, ‡p<0.05 vs. Lean+RAS. ♠ Diet: Significant effect of MetS diet (2-way ANOVA), ♣ Ischemia: Significant effect of ischemia (2-way ANOVA), ♥ Diet x Ischemia: Significant effect of MetS diet x ischemia (2-way ANOVA).