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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: J Mol Biol. 2017 Jun 30;429(16):2542–2555. doi: 10.1016/j.jmb.2017.06.018

Fig. 2.

Fig. 2.

Determining the gag gene and the 5’ UTR sequence important for HIV-1 RNA packaging. (a) General structures of HIV-1 constructs containing a hybrid HIV-1 (NL4–3) gag/syngag gene with a portion of the HIV-1 gag replaced by codon-optimized sequences. Codon optimized sequences are shown as grey boxes. Numbers in the name of each construct indicate the length of NL4–3 gag sequence. (b) RNA genome packaging efficiencies of viral particles generated by HIV-1 constructs containing hybrid gag/syngag genes. Results from six independent experiments are summarized; error bars indicate standard deviations. (c) General structures of HIV-1 constructs containing 5’ UTR deletions. The numbers in the name of each construct indicate deleted sequences; the 1st base of the NL4–3 RNA transcript is defined as 1. (d) RNA genome packaging efficiencies of viral particles generated by HIV-1 constructs with mutations in 5’ UTR. Results from three independent experiments are summarized; error bars indicate the standard deviations.